Paul Evans Library of Fruit Science
Websites on Genetic and Genomic Research on
Grapevine (Vitis spp.)
- The American Vineyard Foundation (AVF) - A California corporation organized in 1978 by the American Society of Enology and Viticulture as a vehicle to raise funds for research in viticulture and enology. Basic and applied research has made the American grape and wine industry the world's leader. Our vineyards produce grapes of unsurpassed quantity and quality.
- Bioinformatics.Org - A clearing house of public resources for plant-focused bioinformatics including plant related database, analysis and presentation technologies. Bioinformatics.Org is a non-profit, academe-based organization committed to opening access to bioinformatics research projects, providing Open Source software for bioinformatics by hosting its development, and keeping biological information freely available. Bioinformatics.Org is a community focused on the freedom of information as it pertains to the biosciences, a community sorely needed in our field, one of the most commercial of all scientific endeavors. And there are no doors to this laboratory to lock out the inquisitive based on any social rank. We stand firmly on our conviction that science is for the sake of science and not to be influenced by political and economic forces.
- Grapevine Genomics at the The Centre for Plant Conservation Genetics: The Grapevine Genome database is a result of a large-scale sequencing project carried out at the Centre for Plant Conservation Genetics. A number of objectives were achieved including the development of SSR markers from grape ESTs, micropropagation of table and wine grape varieties and an analysis of the grape genome based on 5,000 EST sequences. The results of the work on grape ESTs is now available in the Grapevine Genome database.
- The Cooperative Research Centre for Viticulture (CRCV) - The Cooperative Research Centre for Viticulture is a joint venture between Australia's viticulture industry and leading research and education organisations. It promotes cooperative scientific research to accelerate quality viticultural management from vine to palate. Australian grapegrowers and winemakers are key stakeholders in the CRCV, contributing levies matched by the Commonwealth Government and invested by the Grape and Wine Research and Development Corporation in the Centre.
Research Program 3: Molecular Improvement of Grapevines - Molecular biology provides a powerful new approach to understanding biological systems and is resulting in major technological advances in biology, agriculture and medicine. The application of these techniques to grapevines is providing new knowledge that will keep Australian grape and wine industries at the forefront of viticultural science in the future.
This program includes the following projects:
Project 3.4.2: Grapevine gene discovery
Project 3.4.3: Genetic and physical mapping of grapevine genes
Project 3.5 :Adoption of Genetically Modified Grapevines
- Cornell University: Grapevine Breeding and Genetics Program. Research Leader: Bruce Reisch.
- Cornell University: Institute for Genomic Diversity (IGD). Director: Stephen Kresovich.
- CSIRO Plant Industry, Australia - Research Programs - CSIRO applies strategic research in the plant sciences to promote profitable and sustainable agri-food, fibre and horticultural industries, develop novel plant productsand improve natural resource management.
- The Federal Research Station for Plant Production at Changins (RAC), Pully Switzerland, is one of six stations working autonomously within the Agronomic Research Unit of the Federal Office of Agriculture. Its fields of activities cover the whole spectrum of plant production, so in fact it is connected to two centres of competence, one being the Agroecology and Agricultural Research Station in Zurich-Reckenholz (FAL) and the other, the Federal Station for Research in Viticulture, Arboriculture and Horticulture in Wadenswil (FAW) [Competence 2 and Product 21].
- French Institute for Agrononomical Research: INRA (Institut National de Recherche Agronomique) (site is in French)
- Geisenheim Research Centre, Institute for Viticulture and Grapevine Breeding, Germany - This department of the Federal Centre for Breeding Research on Cultivated Plants (BAZ) breeds rootstock and scion varieties and conducts clonal selection of both of them. With rootstocks we aim for a complete Phylloxera resistance in conjunction with high compatibility and adaptation. In regards to cross breeding of scion varieties, the aim is high resistance to downy and powdery mildew in combination with high wine quality. Clonal selection of scion and rootstock varieties aims for high performing clones and the supply of German nurseries with sufficient propagation material. Our major focus are the traditional German varieties.
- Grapevine Biotechnology at the Institute for Wine Biotechnology (IWBT), University of Stellenbosch, South Africa - The IWBT is a member of the "Vitis Microsatellite Consortium" consisting of 20 laboratories world-wide to develop genetic markers, primers and probes for the genetic fingerprinting of Vitis vinifera varieties. Another objective is to establish and optimise efficient transformation and regeneration techniques and procedures. The Grapevine Biotechnology programme includes fundamental studies of gene regulation and expression in grapevine. The overall aim is to develop disease-resistant cultivars so that less pesticides are required in spraying of vineyards. To achieve this goal, it is also necessary to develop tissue-specific gene promoters and convenient cloning vectors and strategies. Molecular genetic techniques are also used to study sucrose metabolism in grapes.
- Institut National de la Recherche Agronomique (INRA)/Centre National Recherche Scientifique (CNRS) - URGV - The Grapevine genome organisation - Introduction: A number of genetic traits are crucial for wine production including quality traits as well as pest resistance. However, the classical analysis of these traits is slowed down by the long generation time of this species. The relatively small size of the the Vitis vinifera genome (470 Mb) compared to other crops makes it an attractive species for genomic approaches. Moreover, Vitis is an orignal and atypical species if compared with already well-studied species from the genomic point of view (Arabidopsis, tomato, rice...).
- International Grape Genomics Initiative - The site (utilizing frames) provides information in the categories: Meetings and Conferences, Grape Experts, Grape Websites, and the Phone Book.
- Institute for Grapevine Breeding, Geilweilerhof, Germany - The institute's research concentrates on: Development of disease-resistant grapevine varieties in consideration of the wide diversity of varieties in German viticulture; Selection methods to assess characteristics such as resistance to noxious agents, resistance to stress factors (e.g. drought, frost), and the flavour and taste-determining aroma components; Collection, maintenance, and evaluation of valuable germplasm of Vitis; Analysis of vine genomes; and Viticulture and Enology Abstracts, supplement to the journal "Vitis - Journal of Grapevine Research" is published by the institute, providing abstracts on grapevine science from scientific literature published throughout the world.
- The Institute of Horticulture of the Agricultural Research Organization, Department of Fruit Tree Breeding & Molecular Genetics - Volcani Center, State of Israel - The Department of Fruit Tree Breeding & Molecular Genetics deals with problems of breeding and selection of cultivars and rootstocks by hybridization and other methods, including tissue and protoplast culture techniques. Breeding of Oleiculture & Viticulture is part of department's activities. Fruit-tree Molecular Genetics research activities are aimed to impruve our basic knowledge of various biological processes on fruit trees, in order to enable improvement of production.
- International Grape Genome Program - The primary research focus is grapevine genomics carried out within the framework of the International Grape Genome Program (IGGP). It is expected that this new genomic knowledge will have a significant impact on our understanding of grapevine biology, viticulture and enology. Plant biologists, viticulturists and enologists worldwide are encouraged to contribute their expertise and form collaborations with genetic and molecular scientists within the IGGP.
- National Center for Biotechnology Information - Established in 1988 as a national resource for molecular biology information, NCBI creates public databases, conducts research in computational biology, develops software tools for analyzing genome data, and disseminates biomedical information - all for the better understanding of molecular processes affecting human health and disease.
- National Clonal Germplasm Repository for Fruit and Nut Crops at Davis, California is one of over two dozen facilities in the National Plant Germplasm System (NPGS) which collect, maintain, characterize, document and distribute plant germplasm from all over the world. It is operated in coordination with the University of California at Davis, and works closely with the UC Davis Department of Pomology, the Department of Viticulture and Enology, and other plant science departments. VITIS: The incredible grape describes the Vitis repository collection, and provides a link to a catalog of available grapes [catalog is in .pdf format].
- National Germplasm Resources System (NPGS) - NPGS is a cooperative effort by public (State and Federal) and private organizations to preserve the genetic diversity of plants. Since many important crop species originate outside the United States, the first steps toward diversity are acquisition and introduction. New germplasm (accessions) enter NPGS through collection, donation by foreign cooperators or international germplasm collections. The accession is then evaluated, maintained, and made available for distribution. Through these efforts, NPGS assists in improving the quality and productivity of crops. The networked data system, or web server, is called commonly GRIN for the Germplasm Resources Information Network.
Search: whereas Taxonomy = Vitis - Selecting a species from the list will lead to another screen providing additional links to the genetic code, the mitochondrial genetic code, and nucleotide and protein strings containing the sequence and mapping data on short genomic landmark sequences or Sequence Tagged Sites.
- National High School of Agronomy of Montpellier: ENSAM (Ecole Nationale Sup=E9rieure d'Agronomie de Montpellier) (site is in French)
- Plant Genetic Resources Unit (PGRU): The USDA - ARS PGRU helps conserve and utilize genetic resources of apple, cold-hardy grape, tart cherry, and certain vegetable crops. The PGRU cooperates with other National Plant Germplasm System units, federal agencies, universities, international research programs, non-governmental organizations, and is located on the Geneva Campus of Cornell University. Vitis is one of the large collections at the PGRU with over 1800 separate accessions of grape are held under commercial field conditions at the Clonal Repository Farm located a mile north of the station.
- The PlantStress web site presents concise and updated discussions of the main stresses: Drought, Heat, Cold (chilling and freezing), Salinity, Soil Mineral Deficiency and Soil Mineral Toxicity. Each discussion is divided into two parts: The Impact of stress; and The Mitigation of the effect of stress.
- Pomology & Viticulture Program at the University of Udine, Italy - The grape research group manages a grape germplasm repository which includes wild species, international and local cultivars and breeding lines carrying disease resistance genes. The group participates to the International VMC (Vitis Microsatellite Consortium) for the development of SSR in grape and to Grape Genome Initiative aiming to develop linkage and physical maps, ESTs, BAC libraries and other tools devoted to understanding grape genome structure and function. Sixty different controlled crosses useful to studying the genetic control of disease resistances and mapping related genes/QTLs have been made involving Asian, American and European genotypes and hybrids.
- Specialized Plant Resources for Informatics and Genomics (SPRIG)
- The "one stop shopping" home page of the worldwide community of plant-focused bioinformatics groups and individuals, who have decided to share bioinformatics resources relating to plants. The site is not species-specific but serves all the plant community: in particular, higher plants of interest either as model organisms (i.e. Arabidopsis thaliana (the general (especially dicotyledon) plant model), Oryza sativa (the crop cereal model) or Medicago truncatula (the legume model)) and other plants of scientific or economic interest. The "SPRIG" initiative and site were set up in mid-August 2001 subsequent to a lunchtime "Birds of a Feather" lunch meeting by a group of plant-focused bioinformaticians at the Intelligent Systems in Molecular Biology (ISMB) 2001 conference in Copenhagen, Denmark. We will likely be affiliated with the proposed "SIGPLANT" special interest group of the (ISCB; International Society for Computational Biology).
- Stress Genomics Consortium - A consortium of laboratories at The University of Arizona, Oklahoma State University, Purdue University and University of Nevada focusing on plant salinity stress. It is our goal to identify all plant genes essential, important or ancillary to salinity stress sensitivity and tolerance.
Tools for Genomics:
Technical Aspects of Microarray Production - As part of the NSF-funded project: Functional Genomics of Stress Tolerance, we have produced separate microarrays from Arabidopsis, ice plant, rice, maize, and barley.
Mutants and Strains - Analysis of mutants (including plant and non-plant models) under stressed and/or non-stressed conditions are presented.
PipeOnline - An online resource for data mining of processed DNA sequence databases.
- The Institute for Genomic Research (TIGR) - The TIGR databases are a collection of curated databases containing DNA and protein sequence, gene expression, cellular role, protein family, and taxonomic data for microbes, plants and humans.
Under Related Web Links are the headings:
Genetics News / Electronic Journals / Genome Centers / DNA Educational Sites / Sequence & Mapping Databases / Government Agencies / Other Databases / Scientific Societies / Software Development Resources / Other Sites
- University of Udine (Italy), Department of Crop Science & Agricultural Engineering (DPVTA) - Research carried out by DPVTA scientists is organized into sections, including the 'Pomology & Viticulture' section with research on grapevine genetics & breeding (genome mapping, breeding for disease resistance, ampelography) .
- Univ. Louis Pasteur, France, the Joint Research Unit for Alsatian Wine and Grapevine, INRA. The Joint Research Unit for Alsatian Wine and Grapevine has been created in Colmar in 2001, in order to focus most of the research topics towards viti-viniculture. This unique structure regroups all of the center's researchers into six teams. The work undertaken aims essentially at genetic improvement of the grapevine, in order to achieve an optimal harvest, leading through its characteristics to the elaboration of a quality wine. Specific research activities and teams are identified.
- Vitis Gene Discovery Program: A Mission to Explore the Genetic Resources of Native North American Grape Species. Wild grapes (Vitis species) are able to thrive in harsh environments and under high disease and pest pressure conditions. Modern viticulture would not be possible without the exploitation of wild grape species native to North America. These grapes are mainly used as rootstocks and breeding germplasm to combat pests, pathogens and environmental stresses. A few of them are also used to make premium regional wines. They are natural sources for genes that confer tolerance to adverse biotic and environmental conditions. We have selected Vitis aestivalis var. Norton and V. cinerea var. Helleri for this program. Project leaders: Dr. Wenping Qiu and Dr. Laszlo Kovacs, Department of Fruit Science, Missouri State University-Mountain Grove.
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- Chardonnay EST Project from ESTAP - ESTAP automatically cleanses raw sequence data by removing vector, low quality, and contaminating sequences. The cleansed sequences are compared to a set of DNA or protein databases using the BLAST algorithms. ESTAP also clusters and assembles EST collections into singlet and contig (redundant) datasets. The raw and cleansed data and analysis results are stored in a relational database. ESTAP affords easy viewing of the original data, the cleansed data, and the analysis results via a Web browser. It also allows the data owner to automatically prepare and submit selected sequences to dbEST. Login instructions are provided.
- EST Database of Grape from Genomics Facility, College of Agricultural and Environmental Sciences, University of California, Davis. - This database contains an analysis of all public expressed sequence tags (ESTs) from grape. ESTs are grouped as contigs or singletons and analyzed for homology to the NCBI Non-Redundant (NR) database by means of BLASTX. The resulting data can be queried by means of unique descriptors (eg. GenBank Numbers, UCD ID Numbers) or based on key words parsed from the NR definition lines of the top hits.
- European Network for Grapevine Genetic Resources Conservation and Characterization - The database is collection-oriented, i.e. the same cultivar/variety appears in the database as many times as there are participating collections containing it. Data (IPGRI passport data, primary and secondary descriptor data) refer to an individual accession (cultivar) only.
- Grape Microsatellite Collection (GMC) - A web-backed database of genotypes at SSR loci obtained from IASMA analysis and literature. GMC is a database developed to permit an easy retrieval of grape nuclear microsatellite profiles and related information. Each record has 8 fields: locus (name of the locus), allele 1 and allele 2 (allele size in bp), cultivar (name of the accession) and finally 3 fields providing information about authors, references and fragment analysis method of collected data.
- Grapevine Genome Database: The Grapevine Genome database is a result of a large-scale sequencing project carried out at the Centre for Plant Conservation Genetics. A number of objectives were achieved including the development of SSR markers from grape ESTs, micropropagation of table and wine grape varieties and an analysis of the grape genome based on 5,000 EST sequences. The results of the work on grape ESTs is now available in the Grapevine Genome database.
- The Greek Vitis Database: A multimedia web-backed genetic database for germplasm management of Vitis resources in Greece. By Francois Lefort and Kalliopi A. Roubelakis-Angelakis, Laboratory of Plant Physiology and Biotechnology,Department of Biology, University of Crete, Heraklion, Crete, Greece.
- Selected GRIN (Germplasm Resources Information Network) search results of the National Germplasm Resources System (NPGS)
Vitis Summary statistics of holdings as of....[Statistics on the holdings of the U.S. National Plant Germplasm System are re-calculated every weekend so the most recent additions may not be included. If you have any questions or suggestions, please contact the site or crop curator.]
Composition for Vitis
GRIN Species of Vitis
Vitis - Grape crop query with the Query conditions: Crop: GRAPE and CORE equals 'Y'
Vitis - Grape listing of holdings in the GRIN database by taxonomy
Vitis - Grape search in the GRIN database based on descriptors
- TIGR Grape Gene Index (VvGI) - The TIGR Grape Gene Index integrates research data from international Grape EST sequencing and gene research projects. The ultimate goal of the TIGR Gene Index projects, including VvGI, is to represent a non-redundant view of all Grape genes and data on their expression patterns, cellular roles, functions, and evolutionary relationships.
- Vitis International Variety Catalogue - All available information has been condensed for each cultivar/variety, i.e. each variety makes a single data set. Data (IPGRI passport data, bibliography, morphological and resistance characteristics and pictures) refer to all informations available for a cultivar.
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Full Articles:
- FAO/IBPGR Technical Guidelines for the Safe Movement of Grapevine Germplasm edited by E. A. Frison and R. Ikin in association with the International Council for the Study of Viruses and Virus Diseases of the Grapevine [in .pdf format].
- Genetic Engineering of Grapevines for Improved Disease Resistance by Julie R. Kikkert and Bruce I. Reisch. 1996. In Grape Research News Vol. 7 No. 2, Summer, 1996. M. Goffinet (ed.). New York State Agricultural Experiment Station, Geneva, New York.
- Genome of Grapevine Culprit Revealed, by Marcia Wood, USDA-ARS, June 26, 2001
- Genomic variability in Vitis vinifera L. "Sangiovese" assessed by microsatellite and non-radioactive AFLP test by Rita Vignani, Monica Scali, Elisa Masi, and Mauro Cresti. Electronic Journal of Biotechnology, Vol. 5 No. 1, Issue of April 15, 2002.
- A Simple and Efficient Method for DNA Extraction from Grapevine Cultivars, Vitis Species and Ampelopsis by Lodhi, Muhammad A., Guang-Ning Ye, Norman F. Weeden and Bruce I. Reisch. 1994. Plant Molecular Biology Reporter. 12(1): 6-13.
- Towards the transcriptome of Grapevine (Vitis vinifera L.) by Albert J. Driesel, Åsa Lommelé, Bernd Drescher, Reinhard Töpfer, Monica Bell, Isabell Cartharius, Natacha Cheutin, Jean-François Huck, Jutta Kubiak, Patric Regnard and André Steinmetz [in .pdf format].
- Transgenic Grapevine Cultivars aimed at Disease Resistance by Melané Vivier, Institute for Wine Biotechnology and Department of Viticulture and Oenology, University of Stellenbosch.
Abstracts:
- Agricultural Microbes Genome 1 Conference, Town & Country Hotel, San Diego, CA, January, 2000 - Abstracts to the conference.
- Agricultural Microbes Genome 2 Conference
January 17-19, 2001 - Abstracts to the conference.
- Breeding Grapevines to Maximize Disease Resistance in New York by R.S. Luce and B.I. Reisch. 1997. Proc. Fourth International Symposium on Cool Climate Viticulture and Enology. 16-20 July 1996. Rochester, New York. (T. Henick-Kling, T.E. Wolf, and E.M. Harkness, eds.) Communication Services, N.Y.S. Agric. Expt. Station, Geneva, NY, USA. pp. VIII-49-51.
- Differences in GUS Expression Among Grapevine Transformants by Michael J. Striem, and B.I. Reisch, and J.R. Kikkert. 1997. Proc. 5th Intern. Symp. Grapevine Physiology. 25-30 May 1997. Jerusalem, Israel. Acta Horticulturae.
- Expression of a Fungal Chitinase in Vitis vinifera L. 'Merlot' and 'Chardonnay' Plants Produced by Biolistic Transformation by J.R. Kikkert, G.M. Reustle, G.S. Ali, P.G. Wallace, and B. I. Reisch. 1998. Proceedings of the VIIth International Symposium on Grapevine Breeding and Genetics, Montpellier, France, July 6-10, 1998. Acta Horticulturae.
- Genetic Engineering of Grapevine (Vitis sp.) for Enhancement of Disease Resistance by Kikkert, J.R., Gul S. Ali, Michael J. Striem, Mary-Howell Martens, Patricia G. Wallace, Linda Molino, and Bruce I. Reisch. 1997. Proc. Third International Symposium on In Vitro Culture and Horticultural Breeding. ISHS. 16-21 June 1996. Jerusalem, Israel (A. Altman and M. Ziv, eds.). Acta Horticulturae. 447:273-279.
- Phosphinothricin Stimulates Somatic Embryogenesis in Grape (Vitis sp. L.) by Dominique Hébert-Soulé, Julie R. Kikkert, and Bruce I. Reisch. Plant Cell Reports. 14:380-384 (1995).
- Transgenic Plantlets of 'Chancellor' Grapevine (Vitis sp.) from Biolistic Transformation of Embryogenic Cell Suspensions by Julie R. Kikkert, Dominique Hébert-Soulé, Patricia G. Wallace, Michael J. Striem and Bruce I. Reisch. 1996. Plant Cell Reports. 15:311-316
Project Summaries:
- Conservation and Utilization of Genetic Resources - Multi-State Research Project Summary Project No: NE-009, Northeastern Regional Association of Agricultural Experiment Station Directors
Program Duration: Initiated 07/53; Current 10/98 to 09/03
Statement of Issues: The nation’s plant germplasm collection is essential for the continued success of conventional and molecular approaches to crop improvement. With the advent of intellectual property rights protection (i.e., patenting, trade secrets) of living organisms, germplasm collections in the public domain have become particularly valuable to research scientists. NE-009 is hosted at the New York State Agricultural Experiment Station in Geneva, NY, and is a component of four plant introduction stations (the others are in Ames IA, Griffin, GA, and Pullman, WA) that make up a national network of public domain collections called the National Plant Germplasm System. NE-009 has the specific mandate to collect, preserve and provide upon request apple, grape, tart cherry, tomato, onion, vegetable Brassica (e.g., cauliflower, cabbage, broccoli, etc.), winter squash, and a few other and approximately 210 other species of plants considered valuable as ornamental, medicinal and food crops. Similar Multi-state Research Projects are operational in the other regions (S-009; W-006; and NC-007). It is through these collections that valuable genetic materials will be preserved, new genetic traits will be identified, improved cultivars will be created, and new agricultural products will be produced.
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- Construction Of Plant Bacterial Artificial Chromosome (BAC) Libraries: An Illustrated Guide by Daniel G. Peterson, Jeffrey P. Tomkins, David A. Frisch, Rod A. Wing, and Andrew H. Paterson
- Grape (Vitis vinifera L.) BAC Library Construction, Preliminary STC Analysis, and Identification of Clones Associated with Flavonoid and Stilbene Biosynthesis by J. P. Tomkins, D.G. Peterson, T.J. Yang, D. Main, E.R Ablett, R.J. Henry, L.S. Lee, T.A. Holton, D. Waters, and R.A. Wing, in Am. J. Enol. Vitic. 52:4 (2001). (in pdf format)
Abstracts:
- A BAC Library for Grape and Strategies for the Development of a Physical Framework for the Grape Genome, by Jeffrey Tomkins, Daniel Peterson, Tae-Jin Yang, Dorrie Main, Effie Ablett, Robert Henry, Slade Lee, Timothy Holton, Dan Waters, Rod Wing. Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002, Town & Country Convention Center, San Diego, CA
- Grape STC Project - Development of Genomic Resources for Grape (Vitis vinifera L. cv. Syrah var. Maxxa): BAC library construction, preliminary STC analysis, and Identification of Clones Associated With Flavonoid and Stilbene Biosynthesis. Clemson University Genomics Institute.
- An INRA BAC libraries platform for gene cloning and genome organization studies of cultivated plants, by Boulos Chalhoub, Agnes Lefevre, Anne-Françoise Adam-Blandon, Harry Belcram, Patricia Rouault, Pascale Laffont, Stephanie Pateyron, Karine Budin, and Michel Caboche, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
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Abstracts:
- Analysis of the Grape Genome, by Robert Henry, Effie Ablett, Slade Lee, Maurizio Rossetto, George Seaton, Michael Staley. Plant & Animal Genome VII Conference, Town & Country Hotel, San Diego, CA, January 17-21, 1999.
- Characterisation and analysis of gene expression during the ripening of grape berries (Vitis vinifera L. cv. Thompson Seedless), by Daniel G. Fischl, Victoria L. Carollo, and Douglas O. Adams, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Grapevine Genomics Access, by Effie Ablett, L Slade Lee, Robert Henry. Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002, Town & Country Convention Center, San Diego, CA
- The Grape Genome Project, by Effie M Ablett, George G Seaton, Kirsten D Scott, Dale A Shelton, L Slade Lee, Guo-Hua Miao, Maureen Dolan, Michael Hanafey, Scott V Tingey, Robert J Henry. Plant & Animal Genome IX Conference, Town & Country Hotel, San Diego, CA, January 13-17, 2001.
- Full length cDNAs of grapevine, Asa Lommele, Natacha Cheutin, Isabell Cartharius, Jean-Francois Huck, Jutta Kubiak, Patric Regnard, Albert J. Driesel, and Andre Steinmetz, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Setting genomics tools sustaining M.A.S. in grapevine (Vitis vinifera L.), by Riccardo Velasco, Giorgia Faes, Rosanna Marino, Marzia Salmaso, Federica Sevini, and M. Stella Grando, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Towards a Better Understanding of Berry Quality and Powdery Mildew Resistance in Grapevine: Genetic Determinism And Pathways, by Agnes Ageorges, Guy Albagnac, Agnes Doligez, Francoise Dosba, Jean-Loup Notteghem, Jean-Pierre Peros, Nancy Terrier, Catherine Tesniere, Patrice This, Laurent Torregrosa Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002, Town & Country Convention Center, San Diego, CA
Project Summaries:
- Functional Genomics of Vitis vinifera. Project Investigators: Grant R. Cramer and John C. Cushman, College of Agriculture, Biotechnology and Natural Resources at the University of Nevada, Reno.
Summary: Abiotic stresses (e.g. cold, heat, salinity, drought) are the major limitation to agricultural productivity and together account for more than half of all production losses. In arid regions of the west, such as the great basin, where crop production is completely dependent on irrigation, greater investments are needed in the development of crops with high water use efficiency and high value-added products. Wine grapes fulfill both of these criteria. Regulated deficit irrigation has been used successfully to grow grapes with less water, an important feature in arid regions such as Nevada. Furthermore, wine grape production under water deficit irrigation regimes has been shown to improve the aroma, flavor and color components of wine by altering metabolite composition, thereby improving wine quality and human health benefits. However, the mechanistic basis for these quality improvements is poorly understood. As a first step toward understanding how water deficits influence the growth of wine grape vines and berries to bring about wine quality improvements following abiotic stress exposure, we have initiated an expressed sequence tag (EST)-based gene discovery program focused solely on stressed vines. We constructed cDNA libraries from mRNA isolated from leaf, root, and berry tissues of Vitis vinifera cv. Chardonnay. Significantly, 42% of the ESTs identified to date have no known function. With our growing database of EST sequence information, we have generated the molecular genetic resources to be able to conduct large-scale gene expression profiling using microarray technology.
Development of cDNA libraries and ESTs: We exposed plants to a variety of abiotic stresses (drought, cold, heat, salt, and flooding; see Table 1 for details) to develop mixed abiotic stress cDNA libraries.
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Full Articles:
Abstracts:
- AFLP: A Powerful Tool To Identify Polymorphisms In Grapevine Among Both Cultivars And Clones, by Ribaut, Jean-MarcelL, Joe Tohme, Patrice This, Eliana Gaitan, Bernard Clot, Enrico Perotti, Philippe Kupfer. Plant & Animal Genome V Conference, Town & Country Hotel, San Diego, CA, January 12-16, 1997.
- Analysis Of Genetic Diversity Between 20 Species Of Vitis Using Fluorescent AFLP Analysis, by Richard A. Descenzo, German Gomez, Warren F. Lamboy, Nancy A. Irelan. Plant & Animal Genome VI Conference, Town & Country Hotel, San Diego, CA, January 18-22, 1998.
- Analysis of the Relationship Between Ancient Grapevine Cultivars Via DNA Fingerprinting by Guang-Ning Ye, Norman F. Weeden, Robert M. Pool and Bruce I. Reisch, Department of Horticultural Sciences, Cornell University, New York State Agricultural Experiment Station, Geneva, NY 14456. Plant Genome II Conference, Town & Country Conference Center, San Diego, CA, January, 1994.
- Analysis of the Relationship Between Grapevine Cultivars, Sports and Clones via DNA Fingerprinting by Guang-Ning Ye, Gökhan Soylemezoglu, Norman F. Weeden, Warren F. Lamboy, Robert M. Pool, and Bruce I. Reisch. 1998. Vitis. 37:33-38.
- The analysis of Vitis chloroplast genome polymorphisms around the mediterranean sea provide clues to understand grapevine domestication, by Rosa Arroyo Garcia, Leonor Ruiz-Garcia, Sabit Agaoglu, Roberto Botta, Felix Cabello, Jose Cenis, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Characterization and Identification of the Portuguese Vitis vinifera Cultivar 'Cruzado de Rabo de Ovelha', using SSR Markers, by Ricardo Magalhaes, Suzana, N. Abreu1, Miguel, A. Faria, Nuno. M. M. Santos, J. E. Eiras Dias, Nuno Magalhaes, Carole, P. Meredith, Francisco Ferreira Monteiro. Plant & Animal Genome VIII Conference, Town & Country Hotel, San Diego, CA, January 9-12, 2000.
- Characterization of New Polymorphic Simple Sequence Repeat Loci and Chloroplast Microsatellites in Grape (Vitis vinifera L.), by Rosa Arroyo-García, Jose Miguel Martínez-Zapater. Plant & Animal Genome VIII Conference, Town & Country Hotel, San Diego, CA, January 9-12, 2000.
- Characterization of RAPD Markers in Vitis by Lodhi, M.A., N.F. Weeden, and B.I. Reisch. 1997. Vitis. 36:133-140
- Comparison of RFLP, AFLP and SSR Markers for Analyzing Phenetic Relationships among Cultivars of Vitis vinifera, by John E. Bowers, Carole P. Meredith. Plant & Animal Genome V Conference, Town & Country Hotel, San Diego, CA, January 12-16, 1997.
- Construction of a Grapevine-Specific Chloroplast Transformation Vector, by Julia Robson, Johan Burger Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002, Town & Country Convention Center, San Diego, CA
- Construction of a Linkage Map for Apirenic Table Grapes and Identification of Markers Linked to Seedlessness, by Kattina Zavala, Nilo Mejia, Boris Sagredo, Patricio Hinrichsen Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002, Town & Country Convention Center, San Diego, CA
- Construction of a Vitis vinifera Linkage Map for the Detection of QTLs of Several Bunch Quality Traits, by Agnès Doligez, Anne-Françoise Adam-Blondon, Jean-François Ballester, Alain Bouquet, Catherine Roux, Patrice This. Plant & Animal Genome VIII Conference, Town & Country Hotel, San Diego, CA, January 9-12, 2000.
- Construction of an AFLP and SSR Genetic Map of Vitis from an Interspecific Hybrid Population, by M. Stella Grando, Diana Bellin, Alberto Madini, Marco Stefanini, Carlo Pozzi, Riccardo Velasco. Plant & Animal Genome VIII Conference, Town & Country Hotel, San Diego, CA, January 9-12, 2000.
- Detection of Quantitative Trait Loci Controlling Important Traits and Characterization of Rapd Markers in Vitis by Muhammad A. Lodhi, Santiago Moreno, Norman F. Weeden and Bruce I. Reisch. Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456. Plant Genome III Conference, Town & Country Conference Center, San Diego, CA, January, 1995.
- A Developing Linkage Map of Vitis vinifera, by Summaira Riaz, Carole Meredith. Plant & Animal Genome VII Conference, Town & Country Hotel, San Diego, CA, January 17-21, 1999.
- Development of a microsatellite-based Vitis vinifera consensus genetic map, by Catherine Roux, Delphine Claux, Gisèle Butterlin, Vincent Chiquet, Stéphane Decroocq, Didier Merdinoglu, Valérie Laucoux, Agnès Doligez, Patrice This, and Anne-Françoise Adam-Blondon, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Development of Grapevine Molecular Markers Based on Retrotransposons, by Frédérique Pelsy, Didier Merdinoglu Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002, Town & Country Convention Center, San Diego, CA
- Full Length cDNAs of Grapevine, by Asa Lommele, Natacha Cheutin, Isabell Cartharius, Jean-Francois Huck , Jutta Kubiak, Patric Regnard, Albert J. Driesel , Andre Steinmetz. Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002, Town & Country Convention Center, San Diego, CA
- Functional genomics of the grape-Xylella interaction: towards the identification of host resistance determinants, by Francisco Goes da Silva, Hyunju Lim, M Andrew Walker, Dongjim Kim, and Douglas R Cook. presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Fungal Disease Resistance in Transgenic Grapevine Expressing a Rice Chitinase, by Toshiya Yamamoto, Hiroyuki Iketani, Hiroyuki Ieki, Yoko Nishizawa, Kazunori Notsuka, Tadaaki Hibi, Tateki Hayashi, Nagao Matsuta. Plant & Animal Genome VII Conference, Town & Country Hotel, San Diego, CA, January 17-21, 1999.
- Gene Controlling Sex in Grapevines Placed on a Molecular Marker-Based Genetic Map by Dalbó, M.A., G.N. Ye, N.F. Weeden, H. Steinkellner, K.M. Sefc, and B.I. Reisch. 2000. Genome 43:333-340.
- Genetic Diversity in Grapevine (Vitis vinifera) Assessed by Microsatellite Markers by Cecile Cuisset, Jean Michel Boursiquot and Patrice This. Plant Genome IV Conference, Town & Country Conference Center, San Diego, CA, January, 1995.
- Genetic Linkage Maps of Vitis and QTL Detection by Interval Mapping by Muhammad A. Lodhi, Bruce I. Reisch and Norman F. Weeden. Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456. Plant Genome II Conference, Town & Country Conference Center, San Diego, CA, January, 1994.
- A genetic map of Vitis rupestris x muscadinia rotundifolia locating resistance to Xiphinema index, the dagger nematode, by Michaeleen Callahan, Yimin Jin, Fangyou Gao, and Andrew Walker, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Genome diversity in cultivated grape, Vitis vinifera L., as revealed by microsatellite polymorphism, by Malli K. Aradhya, Gerald S. Dangl, Bernard H. Prins, and Charles J. Simon, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Genome Diversity in Grapevine as Revealed by SNPs by Salmaso M., Faes G., Segala C., and Velasco R., Istituto Agrario San Michele all’Adige, San Michele a/A (TN), Italy. From the Proceedings of the XLVI Italian Society of Agricultural Genetics - SIGA Annual Congress, Giardini Naxos, Italy - 18/21 September, 2002
- Genotyping Grapevine Cultivars: Towards An International System by M. R. Thomas, P. Cain, N. S. Scott, and James Kijas, CSIRO Division of Horticulture, GPO Box 350, Adelaide, SA 5001. Plant Genome III Conference, Town & Country Conference Center, San Diego, CA, January, 1995.
- Grapevine Functional Genomics, by Mark R. Thomas, Paul K. Boss, Pat Iocco, Chi Hua, Ian B. Dry, Chris Davies Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002, Town & Country Convention Center, San Diego, CA
- Identification and local mapping of resistance gene analogs linked to a powdery mildew resistance locus in grapevine, by Tamzin Donald, Jerôme Pauquet, F Pellerone, Anne-Françoise Adam-Blondon, Alain Bouquet, Mark Thomas, and Ian Dry,presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species by Kristina M. Sefc, Ferdinand Regner, Eva Turetschek, Josef Glössl, and Herta Steinkellner in Genome/Génome 42(3): 367-373 (1999).
- Identification of Markers Linked to the Seedlessness Character in Grapevine, by Florence Lahogue, Patrice This, Anne-Françoise Adam-blondon, Alain Bouquet. Plant & Animal Genome VI Conference, Town & Country Hotel, San Diego, CA, January 18-22, 1998.
- The Interaction of Endochitinase, a Synthetic Peptide ESF12 and Resveratrol in Controlling Fungal Growth in vitro by Ali, G.S., G.E. Harman, and B.I. Reisch. 2000. Phytopathology (submitted)
- Isolation of microsatellites in woody crops, by Guido Cipriani, Gabriele Di Gaspero, Wu-Gang Huang, Giuseppe Lot, M.-Teresa Marrazzo, Rachele Messina, and Raffaele Testolin, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Linkage Map Construction in Two Hybrid Grapevine (Vitis sp.) Populations, by Bruce I. Reisch, N.F. Weeden, M.A. Lodhi, G. Ye and G. Soylemezoglu. Plant Genome IV Conference, Town & Country Conference Center, San Diego, CA, January, 1995.
- Linkage Mapping and QTL Mapping in Grape, by Michael Georgiady, Richard DeScenzo, David W. Cain, Nancy Irelan. Plant & Animal Genome IX Conference, Town & Country Hotel, San Diego, CA, January 13-17, 2001.
- Marker-assisted Selection for Powdery Mildew Resistance in Grapes by Dalbó, M.A., G.N. Ye, N.F. Weeden, W.F. Wilcox, and B.I. Reisch. 2001. Marker-assisted selection for powdery mildew resistance in grapes. J. Amer. Soc. Hort. Sci. 126(1):83-89.
- Microsatellite Length Polymorphism within Ancient Wine Grape Cultivars (Vitis vinifera L.) by John E. Bowers and Carole P. Meredith, Department of Viticulture and Enology, University of California, Davis, CA 95616. Plant Genome II Conference, Town & Country Conference Center, San Diego, CA, January, 1994.
- A Microsatellite Marker Based Linkage Map of Vitis vinifera, Summaira Riaz, Carole P. Meredith. Plant & Animal Genome VIII Conference, Town & Country Hotel, San Diego, CA, January 9-12, 2000.
- Microsatellite Markers for the Differentiation of Clones of Ancient Grape Cultivars, by Summaira Riaz, Keith E. Garrison, Gerald S. Dang, Carole P. Meredith. Plant & Animal Genome IX Conference, Town & Country Hotel, San Diego, CA, January 13-17, 2001.
- Molecular genetic mapping and genome size of Vitis. by Muhammad A. Lodhi, Bruce I. Reisch and Norman F. Weeden, Department of Horticultural Sciences, New York State Agricultural Experiment Station, Cornell University, Geneva, NY 14456, presented at the Plant Genome I Conference, Town & Country Conference Center, San Diego, CA, November, 1992.
- Molecular mapping of grapevine and QTL analysis of fungal disease resistances, by Eva M. Zyprian, Ilkhom Salakhutdinov, Birgitta Fischer, Murat Akkurt, and Reinhard Töpfer, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- A Molecular Marker Based Linkage Map of Vitis by M. A. Lodhi, M. J. Daly, G.-N. Ye, N. F. Weeden and B. I. Reisch. Genome. 38:786-794 (1995).
- Nuclear DNA Content of Vitis Species, Cultivars, and other Genera of the Vitaceae by M.A. Lodhi and B.I. Reisch. Theoretical and Applied Genetics 90:11-16 (1995).
- Prospects for DNA Fingerprinting the Geneva Vitis Genetic Resources Collection using SSR Markers, by Lamboy, Warren Frank. Plant & Animal Genome V Conference, Town & Country Hotel, San Diego, CA, January 12-16, 1997.
- QTL Analysis of Disease Resistance in Interspecific Hybrid Grapes by Dalbó, M.A., G.N. Ye, W.F. Wilcox, N.F. Weeden, and B.I. Reisch. 2000. QTL analysis of disease resistance in interspecific hybrid grapes. (in preparation).
- QTL Analysis of Powdery Mildew Resistance in Grapes by Dalbo, M.A., Ye, G.N. Weeden, N.F., and Reisch, B.I. 1997. Plant and Animal Genome V. 12-16 January 1997, San Diego, CA.
- QTL Analysis of Powdery Mildew Resistance in Grapes, by Dalbó, Marco A., Guang N. Ye, Norman F. Weeden, Bruce I. Reisch. Plant & Animal Genome V Conference, Town & Country Hotel, San Diego, CA, January 12-16, 1997.
- RAPD-Analysis of Different Genotypes of Grape, by Inna Barysheva, Tamara Verbitskaya, Yuri Sivolap. Gene Engineering Department, Plant Breeding and Genetics Institute, Odessa, 270036, Ukraine. Plant Genome III Conference, Town & Country Conference Center, San Diego, CA, January, 1995.
- Setting Genomics Tools Sustaining M.A.S. in Grapevine (Vitis vinifera L.), by Riccardo Velasco, Giorgia Faes, Rosanna Marino, Marzia Salmaso, Federica Sevini, M. Stella Grando. Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002, Town & Country Convention Center, San Diego, CA
- Somatic Stability of Simple Sequence Repeat Loci within Ancient Wine Grape Cultivars, by John Bowers, Rita Vignani and Carole Meredith, Department of Viticulture and Enology, University of California, Davis, California 95616. Plant Genome III Conference, Town & Country Conference Center, San Diego, CA, January, 1995.
- Somatic Variation in Grapevine, by María-Teresa Cervera, Jose-Antonio Cabezas, Jose-Luis Cénis, Jose-Miguel Martínez-Zapater. Plant & Animal Genome VIII Conference, Town & Country Hotel, San Diego, CA, January 9-12, 2000.
- SSR Characterization and Identification of Portuguese Vitis vinifera Cultivars used in Port Wine Production, by Ricardo Magalhães, Miguel Ângelo Faria, Eugénia Nunes, John E. Bowers, Antero Martins, Carole P. Meredith, F. Ferreira Monteiro. Plant & Animal Genome VI Conference, Town & Country Hotel, San Diego, CA, January 18-22, 1998.
- Tagging a Muscadinia rotundifolia-Originated Powdery Mildew Resistance Gene in Vitis vinifera, by Jerome Pauquet, Anne-Francoise Adam-Blondon, Sonia Rousseau, Jean-Pierre Peros, Alain BouquetPlant & Animal Genome VII Conference, Town & Country Hotel, San Diego, CA, January 17-21, 1999.
- Toward the Identification of Grapevine Rootstocks: Development of Molecular Markers based on RAPD, by Patrice This, Cecile Cuisset and Jean-Michel Boursiquot, UFR Viticulture, ENSA.M - INRA, 2 Place Viala, 34060 Montpellier, France. Plant Genome III Conference, Town & Country Conference Center, San Diego, CA, January, 1995.
- Transgenic plants of Vitis vinifera L. cvs. Thompson Seedless and chardonnay expressing the pear PGIP gene,, by Cecilia B Aguero, Abhaya M Dandekar, and Carole P Meredith, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
- Use of microsatellite markers for the analysis of genetic diversity in Vitis vinifera L.: Correlation between molecular and agronomic data, by René Siret, Patrice This, Marc Danzart, and Jean Michel Boursiquot, presented at the Plant, Animal & Microbe Genomes X Conference, January 12-16, 2002
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